||Since the completion of the sequencing of human genome will open up many new areas of study, scientists need to develop new research tools, approaches and capabilities in order to make this vast array of biological information to be useful. The growing availability of the genome sequence data is creating the possibility for developing a comprehensive understanding of complex cellular processes. In order to achieve this goal, it will be necessary to measure multiple parameters on chip in single living cells in real time, for instance, cell impedance, O2, CO2, NO, glucose, pH, and so on. The cell impedance changes when the ion concentration and potential of the cells are different. The impedance for single living cells can be used for fast disease diagnosis.|
This work developed spectroscopy for the single cell impedance measurement. The spectroscopy contains a microfluidic device and an AD5934 Evaluation Board. The microfluidic device captures single cell capture physically by microstructures in the microchannel. The AD5934 Evaluation Board and GUI (Graphic user interface) software could measure the impedance of a single HeLa cell (Human cervical epithelioid carcinoma). The device includes a glass substrate with electrodes and a PDMS channel with micro pillars. The commercial software CFD-ACE+ was used to verify the flow of the microstructures in the channel. Additionally, an equivalent circuit model of the device was established and fitted well to the experimental results in this study.